Construct: shRNA TRCN0000125743

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027189.1-122s1c1
DNA Barcode:
GCTTTGCCTCTGATGGACGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gemin7 (69731)
Gene Description:
gem nuclear organelle associated protein 7
Transcript:
RefSeq NM_027189.1 (NON-CURRENT)
Match location:
Position 122 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000125743)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTGCCTCTGATGGACGTA
Hairpin Sequence:
5'-CCGG-GCTTTGCCTCTGATGGACGTA-CTCGAG-TACGTCCATCAGAGGCAAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTGCCTCTGATGGACGTACTCGAGTACGTCCATCAGAGGCAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTGCCTCTGATGGACGTACTCGAGTACGTCCATCAGAGGCAAAGC-3'

Other clones with same target sequence:

  1. TRCN0000303184