Construct: shRNA TRCN0000125805

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145618.3-457s1c1
DNA Barcode:
GCAGAGAACTTATGTGGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ice2 (93697)
Gene Description:
interactor of little elongation complex ELL subunit 2
Transcript:
RefSeq NM_145618.3 (CURRENT)
Match location:
Position 457 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000125805)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAGAACTTATGTGGATTT
Hairpin Sequence:
5'-CCGG-GCAGAGAACTTATGTGGATTT-CTCGAG-AAATCCACATAAGTTCTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAGAACTTATGTGGATTTCTCGAGAAATCCACATAAGTTCTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAGAACTTATGTGGATTTCTCGAGAAATCCACATAAGTTCTCTGC-3'

Other clones with same target sequence:

  1. TRCN0000312347