Construct: shRNA TRCN0000125826

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010003.1-1145s1c1
DNA Barcode:
CCAAGGGAACAACAGTAGTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyp2c39 (13098)
Gene Description:
cytochrome P450, family 2, subfamily c, polypeptide 39
Transcript:
RefSeq NM_010003.1 (NON-CURRENT)
Match location:
Position 1145 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000125826)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGGGAACAACAGTAGTAA
Hairpin Sequence:
5'-CCGG-CCAAGGGAACAACAGTAGTAA-CTCGAG-TTACTACTGTTGTTCCCTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGGGAACAACAGTAGTAACTCGAGTTACTACTGTTGTTCCCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGGGAACAACAGTAGTAACTCGAGTTACTACTGTTGTTCCCTTGG-3'

Other clones with same target sequence:

(none)