Construct: shRNA TRCN0000125831

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175533.2-293s1c1
DNA Barcode:
GCTGTGGCTCTGTGAAGTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
5830411N06Rik (244234)
Gene Description:
RIKEN cDNA 5830411N06 gene
Transcript:
RefSeq NM_175533.2 (NON-CURRENT)
Match location:
Position 293 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000125831)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTGGCTCTGTGAAGTTTA
Hairpin Sequence:
5'-CCGG-GCTGTGGCTCTGTGAAGTTTA-CTCGAG-TAAACTTCACAGAGCCACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTGGCTCTGTGAAGTTTACTCGAGTAAACTTCACAGAGCCACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTGGCTCTGTGAAGTTTACTCGAGTAAACTTCACAGAGCCACAGC-3'

Other clones with same target sequence:

(none)