Construct: shRNA TRCN0000125842

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028125.1-321s1c1
DNA Barcode:
GCAAGGTCTTCAAGGCACATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zbtb46 (72147)
Gene Description:
zinc finger and BTB domain containing 46
Transcript:
RefSeq NM_028125.1 (NON-CURRENT)
Match location:
Position 321 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000125842)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAGGTCTTCAAGGCACATA
Hairpin Sequence:
5'-CCGG-GCAAGGTCTTCAAGGCACATA-CTCGAG-TATGTGCCTTGAAGACCTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAGGTCTTCAAGGCACATACTCGAGTATGTGCCTTGAAGACCTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAGGTCTTCAAGGCACATACTCGAGTATGTGCCTTGAAGACCTTGC-3'

Other clones with same target sequence:

(none)