Construct: shRNA TRCN0000125893

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010011.2-1495s1c1
DNA Barcode:
CGACTTGTGCTGAAGTCCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyp4a10 (13117)
Gene Description:
cytochrome P450, family 4, subfamily a, polypeptide 10
Transcript:
RefSeq NM_010011.2 (NON-CURRENT)
Match location:
Position 1495 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000125893)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACTTGTGCTGAAGTCCAAA
Hairpin Sequence:
5'-CCGG-CGACTTGTGCTGAAGTCCAAA-CTCGAG-TTTGGACTTCAGCACAAGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACTTGTGCTGAAGTCCAAACTCGAGTTTGGACTTCAGCACAAGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACTTGTGCTGAAGTCCAAACTCGAGTTTGGACTTCAGCACAAGTCG-3'

Other clones with same target sequence:

(none)