Construct: shRNA TRCN0000125897

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_176952.3-328s1c1
DNA Barcode:
ACAACCTTAATCTTCCCTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
6430573F11Rik (319582)
Gene Description:
RIKEN cDNA 6430573F11 gene
Transcript:
RefSeq NM_176952.3 (NON-CURRENT)
Match location:
Position 328 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000125897)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAACCTTAATCTTCCCTTTA
Hairpin Sequence:
5'-CCGG-ACAACCTTAATCTTCCCTTTA-CTCGAG-TAAAGGGAAGATTAAGGTTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAACCTTAATCTTCCCTTTACTCGAGTAAAGGGAAGATTAAGGTTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAACCTTAATCTTCCCTTTACTCGAGTAAAGGGAAGATTAAGGTTGT-3'

Other clones with same target sequence:

(none)