Construct: shRNA TRCN0000125941

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001003947.1-1198s1c1
DNA Barcode:
GCAGGTATGACTGTGGTTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyp4x1 (81906)
Gene Description:
cytochrome P450, family 4, subfamily x, polypeptide 1
Transcript:
RefSeq NM_001003947.1 (CURRENT)
Match location:
Position 1198 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000125941)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGTATGACTGTGGTTCTT
Hairpin Sequence:
5'-CCGG-GCAGGTATGACTGTGGTTCTT-CTCGAG-AAGAACCACAGTCATACCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGTATGACTGTGGTTCTTCTCGAGAAGAACCACAGTCATACCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGTATGACTGTGGTTCTTCTCGAGAAGAACCACAGTCATACCTGC-3'

Other clones with same target sequence:

(none)