Construct: shRNA TRCN0000126166

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018776.1-1001s1c1
DNA Barcode:
CGCTGACATTCAGAGTTGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Crlf3 (54394)
Gene Description:
cytokine receptor-like factor 3
Transcript:
RefSeq NM_018776.1 (NON-CURRENT)
Match location:
Position 1001 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000126166)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTGACATTCAGAGTTGAAA
Hairpin Sequence:
5'-CCGG-CGCTGACATTCAGAGTTGAAA-CTCGAG-TTTCAACTCTGAATGTCAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTGACATTCAGAGTTGAAACTCGAGTTTCAACTCTGAATGTCAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTGACATTCAGAGTTGAAACTCGAGTTTCAACTCTGAATGTCAGCG-3'

Other clones with same target sequence:

  1. TRCN0000308355