Construct: shRNA TRCN0000126198

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175934.2-724s1c1
DNA Barcode:
GCTCGTCAAGTTTATTGATGT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppp1r10 (52040)
Gene Description:
protein phosphatase 1, regulatory subunit 10
Transcript:
RefSeq NM_175934.2 (NON-CURRENT)
Match location:
Position 724 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000126198)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCGTCAAGTTTATTGATGT
Hairpin Sequence:
5'-CCGG-GCTCGTCAAGTTTATTGATGT-CTCGAG-ACATCAATAAACTTGACGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCGTCAAGTTTATTGATGTCTCGAGACATCAATAAACTTGACGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCGTCAAGTTTATTGATGTCTCGAGACATCAATAAACTTGACGAGC-3'

Other clones with same target sequence:

(none)