Construct: shRNA TRCN0000126207

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177863.3-2269s1c1
DNA Barcode:
GCACCTACATTTCCTAGACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Frem1 (329872)
Gene Description:
Fras1 related extracellular matrix protein 1
Transcript:
RefSeq NM_177863.3 (NON-CURRENT)
Match location:
Position 2269 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000126207)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCTACATTTCCTAGACAT
Hairpin Sequence:
5'-CCGG-GCACCTACATTTCCTAGACAT-CTCGAG-ATGTCTAGGAAATGTAGGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCTACATTTCCTAGACATCTCGAGATGTCTAGGAAATGTAGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCTACATTTCCTAGACATCTCGAGATGTCTAGGAAATGTAGGTGC-3'

Other clones with same target sequence:

(none)