Construct: shRNA TRCN0000126228

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029787.2-590s1c1
DNA Barcode:
GTGAAGTCTGTAGGCATGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyb5r3 (109754)
Gene Description:
cytochrome b5 reductase 3
Transcript:
RefSeq NM_029787.2 (CURRENT)
Match location:
Position 590 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000126228)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGAAGTCTGTAGGCATGATT
Hairpin Sequence:
5'-CCGG-GTGAAGTCTGTAGGCATGATT-CTCGAG-AATCATGCCTACAGACTTCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGAAGTCTGTAGGCATGATTCTCGAGAATCATGCCTACAGACTTCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGAAGTCTGTAGGCATGATTCTCGAGAATCATGCCTACAGACTTCAC-3'

Other clones with same target sequence:

(none)