Construct: shRNA TRCN0000126259

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020560.1-989s1c1
DNA Barcode:
CCGAATTCCATGAGCATATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mrps31 (57312)
Gene Description:
mitochondrial ribosomal protein S31
Transcript:
RefSeq NM_020560.1 (NON-CURRENT)
Match location:
Position 989 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000126259)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAATTCCATGAGCATATAT
Hairpin Sequence:
5'-CCGG-CCGAATTCCATGAGCATATAT-CTCGAG-ATATATGCTCATGGAATTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAATTCCATGAGCATATATCTCGAGATATATGCTCATGGAATTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAATTCCATGAGCATATATCTCGAGATATATGCTCATGGAATTCGG-3'

Other clones with same target sequence:

(none)