Construct: shRNA TRCN0000126313

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_134255.2-470s1c1
DNA Barcode:
CGCGGGAGAATCCGATATGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Elovl5 (68801)
Gene Description:
ELOVL family member 5, elongation of long chain fatty acids (yeast)
Transcript:
RefSeq NM_134255.2 (NON-CURRENT)
Match location:
Position 470 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000126313)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCGGGAGAATCCGATATGAA
Hairpin Sequence:
5'-CCGG-CGCGGGAGAATCCGATATGAA-CTCGAG-TTCATATCGGATTCTCCCGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCGGGAGAATCCGATATGAACTCGAGTTCATATCGGATTCTCCCGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCGGGAGAATCCGATATGAACTCGAGTTCATATCGGATTCTCCCGCG-3'

Other clones with same target sequence:

(none)