Construct: shRNA TRCN0000126317

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178751.2-190s1c1
DNA Barcode:
CGGCCACAAGGGCATGGATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Orai2 (269717)
Gene Description:
ORAI calcium release-activated calcium modulator 2
Transcript:
RefSeq NM_178751.2 (NON-CURRENT)
Match location:
Position 190 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000126317)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCCACAAGGGCATGGATTA
Hairpin Sequence:
5'-CCGG-CGGCCACAAGGGCATGGATTA-CTCGAG-TAATCCATGCCCTTGTGGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCCACAAGGGCATGGATTACTCGAGTAATCCATGCCCTTGTGGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCCACAAGGGCATGGATTACTCGAGTAATCCATGCCCTTGTGGCCG-3'

Other clones with same target sequence:

(none)