Construct: shRNA TRCN0000126335

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025911.2-831s1c1
DNA Barcode:
GCGCTGAGCATTATAGTGGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccdc91 (67015)
Gene Description:
coiled-coil domain containing 91
Transcript:
RefSeq NM_025911.2 (CURRENT)
Match location:
Position 831 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000126335)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCTGAGCATTATAGTGGAT
Hairpin Sequence:
5'-CCGG-GCGCTGAGCATTATAGTGGAT-CTCGAG-ATCCACTATAATGCTCAGCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCTGAGCATTATAGTGGATCTCGAGATCCACTATAATGCTCAGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCTGAGCATTATAGTGGATCTCGAGATCCACTATAATGCTCAGCGC-3'

Other clones with same target sequence:

  1. TRCN0000287964