Construct: shRNA TRCN0000126344

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145550.1-1073s1c1
DNA Barcode:
CACAGCATCCACTACTGGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Yipf1 (230584)
Gene Description:
Yip1 domain family, member 1
Transcript:
RefSeq NM_145550.1 (NON-CURRENT)
Match location:
Position 1073 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000126344)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACAGCATCCACTACTGGAAT
Hairpin Sequence:
5'-CCGG-CACAGCATCCACTACTGGAAT-CTCGAG-ATTCCAGTAGTGGATGCTGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACAGCATCCACTACTGGAATCTCGAGATTCCAGTAGTGGATGCTGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACAGCATCCACTACTGGAATCTCGAGATTCCAGTAGTGGATGCTGTG-3'

Other clones with same target sequence:

(none)