Construct: shRNA TRCN0000126399

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013784.1-3207s1c1
DNA Barcode:
GCATCCTGATTCTCCTTCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pign (27392)
Gene Description:
phosphatidylinositol glycan anchor biosynthesis, class N
Transcript:
RefSeq NM_013784.1 (NON-CURRENT)
Match location:
Position 3207 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000126399)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCCTGATTCTCCTTCTTA
Hairpin Sequence:
5'-CCGG-GCATCCTGATTCTCCTTCTTA-CTCGAG-TAAGAAGGAGAATCAGGATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCCTGATTCTCCTTCTTACTCGAGTAAGAAGGAGAATCAGGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCCTGATTCTCCTTCTTACTCGAGTAAGAAGGAGAATCAGGATGC-3'

Other clones with same target sequence:

(none)