Construct: shRNA TRCN0000126505

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175363.2-771s1c1
DNA Barcode:
CCCGAGTCAAACACAGTAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pphln1 (223828)
Gene Description:
periphilin 1
Transcript:
RefSeq NM_175363.2 (NON-CURRENT)
Match location:
Position 771 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000126505)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGAGTCAAACACAGTAGAT
Hairpin Sequence:
5'-CCGG-CCCGAGTCAAACACAGTAGAT-CTCGAG-ATCTACTGTGTTTGACTCGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGAGTCAAACACAGTAGATCTCGAGATCTACTGTGTTTGACTCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGAGTCAAACACAGTAGATCTCGAGATCTACTGTGTTTGACTCGGG-3'

Other clones with same target sequence:

(none)