Construct: shRNA TRCN0000126509

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026617.1-229s1c1
DNA Barcode:
CCTGCCTTAATTGTGGTGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmbim4 (68212)
Gene Description:
transmembrane BAX inhibitor motif containing 4
Transcript:
RefSeq NM_026617.1 (NON-CURRENT)
Match location:
Position 229 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000126509)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCCTTAATTGTGGTGTTT
Hairpin Sequence:
5'-CCGG-CCTGCCTTAATTGTGGTGTTT-CTCGAG-AAACACCACAATTAAGGCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCCTTAATTGTGGTGTTTCTCGAGAAACACCACAATTAAGGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCCTTAATTGTGGTGTTTCTCGAGAAACACCACAATTAAGGCAGG-3'

Other clones with same target sequence:

(none)