Construct: shRNA TRCN0000126562

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011960.1-642s1c1
DNA Barcode:
GCAGTTTCTTACACCTATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Parg (26430)
Gene Description:
poly (ADP-ribose) glycohydrolase
Transcript:
RefSeq NM_011960.1 (NON-CURRENT)
Match location:
Position 642 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000126562)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTTTCTTACACCTATAAA
Hairpin Sequence:
5'-CCGG-GCAGTTTCTTACACCTATAAA-CTCGAG-TTTATAGGTGTAAGAAACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTTTCTTACACCTATAAACTCGAGTTTATAGGTGTAAGAAACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTTTCTTACACCTATAAACTCGAGTTTATAGGTGTAAGAAACTGC-3'

Other clones with same target sequence:

  1. TRCN0000312191