Construct: shRNA TRCN0000126568

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145586.1-323s1c1
DNA Barcode:
GTTCCTGGACAGACATCCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmem159 (233806)
Gene Description:
transmembrane protein 159
Transcript:
RefSeq NM_145586.1 (CURRENT)
Match location:
Position 323 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000126568)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCCTGGACAGACATCCTTT
Hairpin Sequence:
5'-CCGG-GTTCCTGGACAGACATCCTTT-CTCGAG-AAAGGATGTCTGTCCAGGAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCCTGGACAGACATCCTTTCTCGAGAAAGGATGTCTGTCCAGGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCCTGGACAGACATCCTTTCTCGAGAAAGGATGTCTGTCCAGGAAC-3'

Other clones with same target sequence:

(none)