Construct: shRNA TRCN0000126584

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007814.1-2503s1c1
DNA Barcode:
CGGATCTCATAAATACACAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyp2b19 (13090)
Gene Description:
cytochrome P450, family 2, subfamily b, polypeptide 19
Transcript:
RefSeq NM_007814.1 (NON-CURRENT)
Match location:
Position 2503 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000126584)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGATCTCATAAATACACAAA
Hairpin Sequence:
5'-CCGG-CGGATCTCATAAATACACAAA-CTCGAG-TTTGTGTATTTATGAGATCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGATCTCATAAATACACAAACTCGAGTTTGTGTATTTATGAGATCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGATCTCATAAATACACAAACTCGAGTTTGTGTATTTATGAGATCCG-3'

Other clones with same target sequence:

(none)