Construct: shRNA TRCN0000126585

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007814.1-1396s1c1
DNA Barcode:
CCTGAGAACATTGACCTCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyp2b19 (13090)
Gene Description:
cytochrome P450, family 2, subfamily b, polypeptide 19
Transcript:
RefSeq NM_007814.1 (NON-CURRENT)
Match location:
Position 1396 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000126585)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAGAACATTGACCTCATT
Hairpin Sequence:
5'-CCGG-CCTGAGAACATTGACCTCATT-CTCGAG-AATGAGGTCAATGTTCTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAGAACATTGACCTCATTCTCGAGAATGAGGTCAATGTTCTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAGAACATTGACCTCATTCTCGAGAATGAGGTCAATGTTCTCAGG-3'

Other clones with same target sequence:

(none)