Construct: shRNA TRCN0000126608

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139311.1-1271s1c1
DNA Barcode:
CTGAGTCATAAGGACAAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mllt6 (246198)
Gene Description:
myeloid/lymphoid or mixed-lineage leukemia; translocated to, 6
Transcript:
RefSeq NM_139311.1 (NON-CURRENT)
Match location:
Position 1271 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000126608)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGAGTCATAAGGACAAGAAA
Hairpin Sequence:
5'-CCGG-CTGAGTCATAAGGACAAGAAA-CTCGAG-TTTCTTGTCCTTATGACTCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGAGTCATAAGGACAAGAAACTCGAGTTTCTTGTCCTTATGACTCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGAGTCATAAGGACAAGAAACTCGAGTTTCTTGTCCTTATGACTCAG-3'

Other clones with same target sequence:

(none)