Construct: shRNA TRCN0000126613

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199252.1-1275s1c1
DNA Barcode:
GAGCAGCCATTCACTTCTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Unc93a (381058)
Gene Description:
unc-93 homolog A (C. elegans)
Transcript:
RefSeq NM_199252.1 (NON-CURRENT)
Match location:
Position 1275 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000126613)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCAGCCATTCACTTCTCTT
Hairpin Sequence:
5'-CCGG-GAGCAGCCATTCACTTCTCTT-CTCGAG-AAGAGAAGTGAATGGCTGCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCAGCCATTCACTTCTCTTCTCGAGAAGAGAAGTGAATGGCTGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCAGCCATTCACTTCTCTTCTCGAGAAGAGAAGTGAATGGCTGCTC-3'

Other clones with same target sequence:

(none)