Construct: shRNA TRCN0000126724

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181323.2-2505s1c1
DNA Barcode:
GCAATGAGAATCCCATCAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cwh43 (231293)
Gene Description:
cell wall biogenesis 43 C-terminal homolog
Transcript:
RefSeq NM_181323.2 (CURRENT)
Match location:
Position 2505 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000126724)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAATGAGAATCCCATCAATT
Hairpin Sequence:
5'-CCGG-GCAATGAGAATCCCATCAATT-CTCGAG-AATTGATGGGATTCTCATTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAATGAGAATCCCATCAATTCTCGAGAATTGATGGGATTCTCATTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAATGAGAATCCCATCAATTCTCGAGAATTGATGGGATTCTCATTGC-3'

Other clones with same target sequence:

(none)