Construct: shRNA TRCN0000126757

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053014.2-604s1c1
DNA Barcode:
GATCGTATTCTGCAAACGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Agpat3 (28169)
Gene Description:
1-acylglycerol-3-phosphate O-acyltransferase 3
Transcript:
RefSeq NM_053014.2 (NON-CURRENT)
Match location:
Position 604 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000126757)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCGTATTCTGCAAACGGAA
Hairpin Sequence:
5'-CCGG-GATCGTATTCTGCAAACGGAA-CTCGAG-TTCCGTTTGCAGAATACGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCGTATTCTGCAAACGGAACTCGAGTTCCGTTTGCAGAATACGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCGTATTCTGCAAACGGAACTCGAGTTCCGTTTGCAGAATACGATC-3'

Other clones with same target sequence:

(none)