Construct: shRNA TRCN0000126786

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172051.2-1503s1c1
DNA Barcode:
CTGTGACTCTTCTCGCCATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmcc3 (319880)
Gene Description:
transmembrane and coiled coil domains 3
Transcript:
RefSeq NM_172051.2 (NON-CURRENT)
Match location:
Position 1503 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000126786)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTGACTCTTCTCGCCATAT
Hairpin Sequence:
5'-CCGG-CTGTGACTCTTCTCGCCATAT-CTCGAG-ATATGGCGAGAAGAGTCACAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTGACTCTTCTCGCCATATCTCGAGATATGGCGAGAAGAGTCACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTGACTCTTCTCGCCATATCTCGAGATATGGCGAGAAGAGTCACAG-3'

Other clones with same target sequence:

(none)