Construct: shRNA TRCN0000126820

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181853.2-3827s1c1
DNA Barcode:
CCAGGCATTATTACCAGATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trim66 (330627)
Gene Description:
tripartite motif-containing 66
Transcript:
RefSeq NM_181853.2 (NON-CURRENT)
Match location:
Position 3827 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000126820)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGCATTATTACCAGATTA
Hairpin Sequence:
5'-CCGG-CCAGGCATTATTACCAGATTA-CTCGAG-TAATCTGGTAATAATGCCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGCATTATTACCAGATTACTCGAGTAATCTGGTAATAATGCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGCATTATTACCAGATTACTCGAGTAATCTGGTAATAATGCCTGG-3'

Other clones with same target sequence:

(none)