Construct: shRNA TRCN0000126884

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_206869.1-108s1c1
DNA Barcode:
CCATAATTTCTATCCAGTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vmn1r180 (232962)
Gene Description:
vomeronasal 1 receptor 180
Transcript:
RefSeq NM_206869.1 (NON-CURRENT)
Match location:
Position 108 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000126884)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATAATTTCTATCCAGTCTT
Hairpin Sequence:
5'-CCGG-CCATAATTTCTATCCAGTCTT-CTCGAG-AAGACTGGATAGAAATTATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATAATTTCTATCCAGTCTTCTCGAGAAGACTGGATAGAAATTATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATAATTTCTATCCAGTCTTCTCGAGAAGACTGGATAGAAATTATGG-3'

Other clones with same target sequence:

  1. TRCN0000202664