Construct: shRNA TRCN0000126948

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015822.1-1394s1c1
DNA Barcode:
GCCTCTTGATGAAGAGTTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fbxl3 (50789)
Gene Description:
F-box and leucine-rich repeat protein 3
Transcript:
RefSeq NM_015822.1 (NON-CURRENT)
Match location:
Position 1394 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000126948)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCTTGATGAAGAGTTAAT
Hairpin Sequence:
5'-CCGG-GCCTCTTGATGAAGAGTTAAT-CTCGAG-ATTAACTCTTCATCAAGAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCTTGATGAAGAGTTAATCTCGAGATTAACTCTTCATCAAGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCTTGATGAAGAGTTAATCTCGAGATTAACTCTTCATCAAGAGGC-3'

Other clones with same target sequence:

(none)