Construct: shRNA TRCN0000126971

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_134144.1-324s1c1
DNA Barcode:
CTAATGGAATGGGCATTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyp2c50 (107141)
Gene Description:
cytochrome P450, family 2, subfamily c, polypeptide 50
Transcript:
RefSeq NM_134144.1 (NON-CURRENT)
Match location:
Position 324 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000126971)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAATGGAATGGGCATTATAT
Hairpin Sequence:
5'-CCGG-CTAATGGAATGGGCATTATAT-CTCGAG-ATATAATGCCCATTCCATTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAATGGAATGGGCATTATATCTCGAGATATAATGCCCATTCCATTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAATGGAATGGGCATTATATCTCGAGATATAATGCCCATTCCATTAG-3'

Other clones with same target sequence:

(none)