Construct: shRNA TRCN0000127010
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_153589.1-1511s1c1
- DNA Barcode:
- GCCTCCATCTTGTTTATGATT
Original Target:
- Taxon:
- Mus musculus (mouse)
- Gene:
- Ano2 (243634)
- Gene Description:
- anoctamin 2
- Transcript:
- RefSeq NM_153589.1 (NON-CURRENT)
- Match location:
- Position 1511 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000127010)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
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Sequence Information
- Target Sequence:
- GCCTCCATCTTGTTTATGATT
- Hairpin Sequence:
- 5'-CCGG-GCCTCCATCTTGTTTATGATT-CTCGAG-AATCATAAACAAGATGGAGGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCTCCATCTTGTTTATGATTCTCGAGAATCATAAACAAGATGGAGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCTCCATCTTGTTTATGATTCTCGAGAATCATAAACAAGATGGAGGC-3'