Construct: shRNA TRCN0000127082

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028946.1-1031s1c1
DNA Barcode:
CCACCTCTCATTGGGATGTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc9b1 (74446)
Gene Description:
solute carrier family 9, subfamily B (NHA1, cation proton antiporter 1), member 1
Transcript:
RefSeq NM_028946.1 (NON-CURRENT)
Match location:
Position 1031 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127082)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCTCTCATTGGGATGTTA
Hairpin Sequence:
5'-CCGG-CCACCTCTCATTGGGATGTTA-CTCGAG-TAACATCCCAATGAGAGGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCTCTCATTGGGATGTTACTCGAGTAACATCCCAATGAGAGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCTCTCATTGGGATGTTACTCGAGTAACATCCCAATGAGAGGTGG-3'

Other clones with same target sequence:

(none)