Construct: shRNA TRCN0000127101

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024196.1-658s1c1
DNA Barcode:
GCACATCCTAAATTATCTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tbc1d20 (67231)
Gene Description:
TBC1 domain family, member 20
Transcript:
RefSeq NM_024196.1 (NON-CURRENT)
Match location:
Position 658 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127101)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACATCCTAAATTATCTGAT
Hairpin Sequence:
5'-CCGG-GCACATCCTAAATTATCTGAT-CTCGAG-ATCAGATAATTTAGGATGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACATCCTAAATTATCTGATCTCGAGATCAGATAATTTAGGATGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACATCCTAAATTATCTGATCTCGAGATCAGATAATTTAGGATGTGC-3'

Other clones with same target sequence:

  1. TRCN0000325371