Construct: shRNA TRCN0000127127

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019959.1-570s1c1
DNA Barcode:
GAACCTCTACAAACACTTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
C1qtnf1 (56745)
Gene Description:
C1q and tumor necrosis factor related protein 1
Transcript:
RefSeq NM_019959.1 (NON-CURRENT)
Match location:
Position 570 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127127)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACCTCTACAAACACTTCAA
Hairpin Sequence:
5'-CCGG-GAACCTCTACAAACACTTCAA-CTCGAG-TTGAAGTGTTTGTAGAGGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACCTCTACAAACACTTCAACTCGAGTTGAAGTGTTTGTAGAGGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACCTCTACAAACACTTCAACTCGAGTTGAAGTGTTTGTAGAGGTTC-3'

Other clones with same target sequence:

(none)