Construct: shRNA TRCN0000127146

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001011873.1-1064s1c1
DNA Barcode:
GCCCAAACTCATGTATCTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Xkr9 (381246)
Gene Description:
X-linked Kx blood group related 9
Transcript:
RefSeq NM_001011873.1 (NON-CURRENT)
Match location:
Position 1064 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127146)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAAACTCATGTATCTCTT
Hairpin Sequence:
5'-CCGG-GCCCAAACTCATGTATCTCTT-CTCGAG-AAGAGATACATGAGTTTGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAAACTCATGTATCTCTTCTCGAGAAGAGATACATGAGTTTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAAACTCATGTATCTCTTCTCGAGAAGAGATACATGAGTTTGGGC-3'

Other clones with same target sequence:

(none)