Construct: shRNA TRCN0000127152

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027126.2-1014s1c1
DNA Barcode:
GCTCGAGTTTGTCCATTCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hfe2 (69585)
Gene Description:
hemochromatosis type 2 (juvenile)
Transcript:
RefSeq NM_027126.2 (NON-CURRENT)
Match location:
Position 1014 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127152)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCGAGTTTGTCCATTCAAA
Hairpin Sequence:
5'-CCGG-GCTCGAGTTTGTCCATTCAAA-CTCGAG-TTTGAATGGACAAACTCGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCGAGTTTGTCCATTCAAACTCGAGTTTGAATGGACAAACTCGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCGAGTTTGTCCATTCAAACTCGAGTTTGAATGGACAAACTCGAGC-3'

Other clones with same target sequence:

(none)