Construct: shRNA TRCN0000127170

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010005.2-833s1c1
DNA Barcode:
GAGAATCTGTTGACTGAGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyp2d10 (13101)
Gene Description:
cytochrome P450, family 2, subfamily d, polypeptide 10
Transcript:
RefSeq NM_010005.2 (NON-CURRENT)
Match location:
Position 833 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127170)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAATCTGTTGACTGAGAAT
Hairpin Sequence:
5'-CCGG-GAGAATCTGTTGACTGAGAAT-CTCGAG-ATTCTCAGTCAACAGATTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAATCTGTTGACTGAGAATCTCGAGATTCTCAGTCAACAGATTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAATCTGTTGACTGAGAATCTCGAGATTCTCAGTCAACAGATTCTC-3'

Other clones with same target sequence:

(none)