Construct: shRNA TRCN0000127213

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008566.1-1623s1c1
DNA Barcode:
TGATATGATCTTCATCGTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mcm5 (17218)
Gene Description:
minichromosome maintenance complex component 5
Transcript:
RefSeq NM_008566.1 (NON-CURRENT)
Match location:
Position 1623 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127213)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATATGATCTTCATCGTCAA
Hairpin Sequence:
5'-CCGG-TGATATGATCTTCATCGTCAA-CTCGAG-TTGACGATGAAGATCATATCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATATGATCTTCATCGTCAACTCGAGTTGACGATGAAGATCATATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATATGATCTTCATCGTCAACTCGAGTTGACGATGAAGATCATATCA-3'

Other clones with same target sequence:

  1. TRCN0000312209