Construct: shRNA TRCN0000127254

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025943.2-3319s1c1
DNA Barcode:
GCCGTTACTAACGAGTTCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dzip1 (66573)
Gene Description:
DAZ interacting protein 1
Transcript:
RefSeq NM_025943.2 (NON-CURRENT)
Match location:
Position 3319 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127254)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGTTACTAACGAGTTCTTT
Hairpin Sequence:
5'-CCGG-GCCGTTACTAACGAGTTCTTT-CTCGAG-AAAGAACTCGTTAGTAACGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGTTACTAACGAGTTCTTTCTCGAGAAAGAACTCGTTAGTAACGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGTTACTAACGAGTTCTTTCTCGAGAAAGAACTCGTTAGTAACGGC-3'

Other clones with same target sequence:

(none)