Construct: shRNA TRCN0000127261

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_134167.1-181s1c1
DNA Barcode:
CTTACAGATTTCTTTGTGTCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vmn1r35 (171185)
Gene Description:
vomeronasal 1 receptor 35
Transcript:
RefSeq NM_134167.1 (CURRENT)
Match location:
Position 181 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127261)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTACAGATTTCTTTGTGTCA
Hairpin Sequence:
5'-CCGG-CTTACAGATTTCTTTGTGTCA-CTCGAG-TGACACAAAGAAATCTGTAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTACAGATTTCTTTGTGTCACTCGAGTGACACAAAGAAATCTGTAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTACAGATTTCTTTGTGTCACTCGAGTGACACAAAGAAATCTGTAAG-3'

Other clones with same target sequence:

(none)