Construct: shRNA TRCN0000127274

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173453.1-932s1c1
DNA Barcode:
CCTGGGAAAGTGTTTGGTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmem11 (216821)
Gene Description:
transmembrane protein 11
Transcript:
RefSeq NM_173453.1 (NON-CURRENT)
Match location:
Position 932 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127274)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGGAAAGTGTTTGGTTTA
Hairpin Sequence:
5'-CCGG-CCTGGGAAAGTGTTTGGTTTA-CTCGAG-TAAACCAAACACTTTCCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGGAAAGTGTTTGGTTTACTCGAGTAAACCAAACACTTTCCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGGAAAGTGTTTGGTTTACTCGAGTAAACCAAACACTTTCCCAGG-3'

Other clones with same target sequence:

  1. TRCN0000331617