Construct: shRNA TRCN0000127289

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172923.1-2195s1c1
DNA Barcode:
CCAAGCCGTCTTTCCAGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmem266 (244886)
Gene Description:
transmembrane protein 266
Transcript:
RefSeq NM_172923.1 (NON-CURRENT)
Match location:
Position 2195 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127289)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGCCGTCTTTCCAGAAAT
Hairpin Sequence:
5'-CCGG-CCAAGCCGTCTTTCCAGAAAT-CTCGAG-ATTTCTGGAAAGACGGCTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGCCGTCTTTCCAGAAATCTCGAGATTTCTGGAAAGACGGCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGCCGTCTTTCCAGAAATCTCGAGATTTCTGGAAAGACGGCTTGG-3'

Other clones with same target sequence:

(none)