Construct: shRNA TRCN0000127313

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027920.3-562s1c1
DNA Barcode:
CCATGAGTCATCCAAGCAACA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
March8 (71779)
Gene Description:
membrane-associated ring finger (C3HC4) 8
Transcript:
RefSeq NM_027920.3 (NON-CURRENT)
Match location:
Position 562 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127313)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGAGTCATCCAAGCAACA
Hairpin Sequence:
5'-CCGG-CCATGAGTCATCCAAGCAACA-CTCGAG-TGTTGCTTGGATGACTCATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGAGTCATCCAAGCAACACTCGAGTGTTGCTTGGATGACTCATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGAGTCATCCAAGCAACACTCGAGTGTTGCTTGGATGACTCATGG-3'

Other clones with same target sequence:

(none)