Construct: shRNA TRCN0000127327

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177224.1-4266s1c1
DNA Barcode:
GCAGTTAAAGTCTACAGATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Chd9 (109151)
Gene Description:
chromodomain helicase DNA binding protein 9
Transcript:
RefSeq NM_177224.1 (NON-CURRENT)
Match location:
Position 4266 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127327)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTTAAAGTCTACAGATTA
Hairpin Sequence:
5'-CCGG-GCAGTTAAAGTCTACAGATTA-CTCGAG-TAATCTGTAGACTTTAACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTTAAAGTCTACAGATTACTCGAGTAATCTGTAGACTTTAACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTTAAAGTCTACAGATTACTCGAGTAATCTGTAGACTTTAACTGC-3'

Other clones with same target sequence:

(none)