Construct: shRNA TRCN0000127336

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_194055.1-929s1c1
DNA Barcode:
CCTCCCATTATCCCTGTACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Esrp1 (207920)
Gene Description:
epithelial splicing regulatory protein 1
Transcript:
RefSeq NM_194055.1 (NON-CURRENT)
Match location:
Position 929 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127336)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCCATTATCCCTGTACTA
Hairpin Sequence:
5'-CCGG-CCTCCCATTATCCCTGTACTA-CTCGAG-TAGTACAGGGATAATGGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCCATTATCCCTGTACTACTCGAGTAGTACAGGGATAATGGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCCATTATCCCTGTACTACTCGAGTAGTACAGGGATAATGGGAGG-3'

Other clones with same target sequence:

(none)