Construct: shRNA TRCN0000127343

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178390.2-1225s1c1
DNA Barcode:
CCTGGACTATGAAGACGATGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam53a (74504)
Gene Description:
family with sequence similarity 53, member A
Transcript:
RefSeq NM_178390.2 (NON-CURRENT)
Match location:
Position 1225 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127343)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGACTATGAAGACGATGA
Hairpin Sequence:
5'-CCGG-CCTGGACTATGAAGACGATGA-CTCGAG-TCATCGTCTTCATAGTCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGACTATGAAGACGATGACTCGAGTCATCGTCTTCATAGTCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGACTATGAAGACGATGACTCGAGTCATCGTCTTCATAGTCCAGG-3'

Other clones with same target sequence:

(none)