Construct: shRNA TRCN0000127383

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008579.4-375s1c1
DNA Barcode:
GCAGGATATCGGGATGAAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Meig1 (104362)
Gene Description:
meiosis expressed gene 1
Transcript:
RefSeq NM_008579.4 (CURRENT)
Match location:
Position 375 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127383)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGATATCGGGATGAAATT
Hairpin Sequence:
5'-CCGG-GCAGGATATCGGGATGAAATT-CTCGAG-AATTTCATCCCGATATCCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGATATCGGGATGAAATTCTCGAGAATTTCATCCCGATATCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGATATCGGGATGAAATTCTCGAGAATTTCATCCCGATATCCTGC-3'

Other clones with same target sequence:

(none)